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pCAG-EGFP-Ube3C C1051S The EGFP fusion protein was created by amplification of respective templates (pCAG-myc-Ube3C-C1051S and pCAG-eGFP, Addgene, #89684) using the following oligos: 5’-tgtctcatcattttggcaaagATGGTGAGCAAGGGCGAGGAG-3’ with 5’-tcgaagctgaaCTTGTACAGCTCGTCCATGCC-3’ and 5’-gctgtacaagTTCAGCTTCGAAGGCGACTTC-3’ with 5’-gcggccgcgatatcctcgaggTCAGCTCAGCTCAAAGCCAGC-3’ and inserted into the pCAG-expression vector (Addgene, #11160) using EcoRI restriction and NEBuilder system

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“One of the most common complications that we see is infection, usually at the site of the IV placement,” she said