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Eluate was concentrated using an Amicon Ultra (MWCO 100K, Millipore) centrifugal device and then purified by Superose 6 Increase 10/300 column (GE Healthcare) in buffer containing 50 mM HEPES pH 8.0, 150 mM KCl, 0.05% DDM, 0.01% CHS, and 1 mM DTT

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