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the treatment target of tumor obstacle The invention provides the method for the treatment target that is used to identify the tumor obstacle.The present invention also provides the treatment target of identifying through these class methods.The evaluation of treatment target generally includes and one group of cell or cell line external source environment influences agent or alternative circumstances influence agent and assessment subsequently and contrast untreated cell and compare to the variation of the cell induction of processing.Monitored inductive cellular change includes but not limited to the change to morphology, physiology or composition, for example the level of the RNA of cell, protein, lipid or metabolite.Result's inductive cellular change as utilizing alternative circumstances to influence the treatment of agent can be monitored through using any algoscopy of describing among this paper.For example, the variation of PCR in real time array assessment gene expression capable of using on the mRNA level, and the variation of gene expression on protein level can be monitored through using antibody microarray and 2-D gel electrophoresis.Use path analysis (Ingenuity IPA software) subsequently and be accredited as from the assessment of systems biology angle and receive alternative circumstances to influence the gene that (for example, on mRNA and/or protein level) regulated in agent through looking back known references.The gene experience checking algoscopy that then will be accredited as potential treatment target for example Western engram analysis, siRNA is struck the generation and the characterizing method of low or recombinant protein.Subsequently the Screening test method is used to identify the regulator of target.The regulator of treatment target is as the new therapeutic agent of tumor obstacle.Can use the Screening test method described in detail among this paper or through using conventional method well known by persons skilled in the art to identify the regulator of treatment target routinely
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24, 400–406 [DOI] [PMC free article] [PubMed] [Google Scholar] 175.Dibble C.C
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