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Here, we have used a modified hemoglobin fractionation assay 32, 34 in 3-6 hpi ring-and 24 hpi trophozoite-stage artemisinin resistant (Cam3.II R539T , Cam3.II C580Y and MRA1240) and sensitive (Cam3.II REV and MRA1239) parasites to measure hemoglobin, heme and hemozoin species

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Incubate the plate at room temperature for 30 min to allow the reaction to occur