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Cuprizone induces mitochondrial injury and oxidative stress by increasing mitochondrial production of superoxide anions 4 , reducing copper-dependent cytochrome c oxidase and monoamine oxidase activity 1 , decreasing copper-zinc superoxide dismutase 5 and glutathione 6 antioxidant activity, and inhibiting electron transport chain complex IV 7

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These findings were indicative of increased oxidative damage