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Behavioral testing was followed by biochemical analyses on colon, faces, serum, cerebellum, and prefrontal cortex, assessing tryptophan metabolites, serotonin (5-HT) pathway intermediates, neurotransmitters (GABA, glutamate, dopamine, acetylcholine) via UPLC, UPLC-TQD, and ELISA, alongside gut microbiota profiling through 16S r RNA sequencing

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In light of the “amyloid cascade hypothesis” considerable research effort has focused on the accumulation of insoluble brain Aβ protein as a basic cause of AD [47,48]