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Previous studies have also shown that cell lines like MDA-MB-468, which express high levels of PHGDH and have activated SSP, are sensitive to sh-RNA mediated depletion of PHGDH, regardless of the presence of serine in the medium 14,15 , indicating a difference between loss and inhibition of PHGDH

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b , Left, lower magnification of the images in panel a (scale bar, 50 μm)