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Chemical reagents For some experiments, keratinocytes were treated with either dimethyl sulfoxide (DMSO) (Sigma-Aldrich) or 100 µM CK666 (Sigma-Aldrich) for 4 h to inhibit Arp2/3 complex activity

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It has been suggested that the loss of Ca 2+ in macrophages containing MTB phagosomes reduces phagosome-lysosome fusion to some extent [105]