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Hsp90 is critical for eNOS activity, and is involved in a reciprocal interactive relationship with eNOS and CaM : Hsp90 enhances both the magnitude and sensitivity of eNOS activation in response to Ca 2+ -CaM

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The pH of the media was adjusted to pH 6.5 and sterilized by filtration through a sterile filter with a pore size of 0.2-μm (Gelman Sciences, Ann Arbor, MI, United States)