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CJC-1295 Peptide: Structure, Benefits, and Research Use

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2D-gel electrophoresis and mass spectrometry Aliquots containing 100 mg of protein extracts from treated leaves were precipitated using TCA in acetone and then redissolved in a reswelling buffer consisting of 7 M urea, 2 M thiourea, 2% (v/v) CHAPS, 0.5% (v/v) IPG ampholytes (pH 4–7, Thermo Fisher Scientific, USA), 1.2% (v/v) Destreak reagent (hydroxyethyl disulfide), and a small amount of bromophenol blue