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This technique can include several rounds of centrifugation with different speeds and durations and can include sucrose gradients to achieve a desired cellular component (316–318)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Shapiro T.A., Fahey J.W., Dinkova-Kostova A.T., Holtzclaw W.D., Stephenson K.K., Wade K.L., Ye L., Talalay P