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Then, they were incubated with primary antibodies for TOM20 (Santa Cruz Biotechnology, Heidelberg, Germany, 1:1000 dilution), p-mTOR Ser2448 (Cell Signaling Technology, Leiden, Netherlands, 1:1000), mTOR (Cell Signaling Technology, Leiden, Netherlands, 1:1000), p-ULK Ser757 (Cell Signaling Technology, Leiden, Netherlands, 1:1000), ULK (Cell Signaling Technology, Leiden, Netherlands, 1:1000), Beclin-1 (Santa Cruz Biotechnology, Heidelberg, Germany, 1:1000), LC3-II (Sigma, 1:5000)