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A standard volume of master mix was added to each reaction tube, and Ub was added to initiate the ubiquitination reaction containing 100µM Ub, 1µM SKP1-FBXO22, 0.5µM *NSD2 PWWP1, 0.5µM compound, 0.1µM UBA1, 1µM UBE2R2, 1µM UBE2D2 or 1µM UBE2L3, and 1µM ARIH1, 1µM NEDD8~CUL1-RBX1, and 5mM MgATP in reaction buffer

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436 Acting as a soluble decoy receptor, it captures both IL-1α and IL-1β