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Concentration then rests on peptide-bond absorbance in the far ultraviolet, which is matrix-sensitive and demands a matched standard, or on amino acid analysis, or in practice on mass-by-difference: weigh the vial contents, assume the labeled mass is peptide, divide by volume

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Meta‐regression against age, male, BMI, and duration did not reveal statistically significant differences across treatment groups for any of the evaluated characteristics, indicating that the transitivity assumption was reasonably upheld