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TVE was also unsuccessful: both pts morbidly obese, wheelchair bound and had ascites

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Results clearly showed that the tyrosine-nitration level was reduced in proportion to the molar ratios of GSSSG and LASSS relative to HGF, as revealed by the decrease in anti-nitroY mAb-positive HGF (60-kDa α-chain and 90-kDa proform) normalized to total HGF α-chain as a loading control, that was monitored by stripping with SDS-βME solution and re-probing the blot with anti-HGF α-chain N-terminal domain mAb (Figure 1b,c, second and third rows)

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