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Then, RNF213 -knockout HeLa cells were transfected with relevant passer transposon system to generate a stable cell line expressing AcGFP-tagged wild-type RNF213 (Supplementary Fig
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The first signal in this activation process seems to rely on the production of mitochondrial reactive oxygen species (ROS) and the upregulation of NLRP3 expression through signaling pathways involving Toll-like receptors (TLR) and myeloid differentiation response (MyD88) nuclear factors κB (NF-κB) (13)
Gently massage and roll in a circular motion until enough dead skin cells have visibly lifted