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To quantify the frequency of interaction between GSSG functional groups and protein residues, the pairwise distances between all GSSG functional group atoms and protein atoms were calculated for each trajectory frame

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Similarly, genetic deletion of floxed Gpx4 in Pfa1 cells 22 increased endogenous S 0 levels, including glutathione hydropersulfide and hydrotrisulfide (GSSH and GSSSH, respectively) (Fig