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Derivatization methods HPLC-MS (or HPLC-MS/MS) detection is often used for the determination of compounds in biological samples, but the sensitivity of this method can be inadequate due to low ionization efficiency of the analyzed specimen

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The rationale behind this study is based on the promising anti-inflammatory and protease inhibitory properties demonstrated by Minocycline in various preclinical stroke models (Sheng et al., 2018)