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For cytotoxicity assays, cells were subcultured and seeded at 3000 cells/well in 96-well flat plates and grown to optimal confluency in 5 days

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The convective-diffusive mixing of two miscible NPs precursor solutions (a DPPC:cholesterol: dicetylphosphate (DCP) phospholipid formulation in isopropanol and a photopolymerizable N-isopropyl acrylamide combination in aqueous buffer) was managed by researchers adopting microfluidic hydrodynamic focusing