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IC50 values (the concentration required to inhibit 50% of enzyme activity) for 5-Amino-1MQ against NNMT are in the low micromolar range (approximately 2–5 µM in published in vitro assays), while IC50 values for structurally related methyltransferases are orders of magnitude higher, suggesting high specificity

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Ferritin is unreliable in cases of inflammation therefore transferrin saturation is more reliable but has a 2 hour window for testing after blood draw for accurate results