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From GLP-1 medications for diabetes and weight loss to growing interest in experimental compounds promoted for recovery and anti-aging, peptides are attracting unprecedented attention. Recent FDA discussions have also put the spotlight

However, it should be noted that ascorbate may also act as a prooxidant in vivo

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In practice, the pH is typically at least 1 unit higher or lower than pI of target protein to ensure the protein has an expected surface charge