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Genistein promotes proliferation of human cervical cancer cells through estrogen receptor-mediated Pi3k/Akt-Nf-Κb pathway

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Alkylated proteins were incubated with either 10 μg trypsin (4 h at 37°C) in ABC buffer, or 10 μg pepsin (1 h at 37°C) with added 1% formic acid in 10% MeOH to obtain pH 2 during digestion