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Establishing effective concentrations of nicotine For the purpose of establishing effective concentrations of nicotine, incubations were performed with confluent monolayer cultures of 4 of the established cell-lines of HGF in Eagle's MEM using 14C-testosterone/14C-4-androstenedione as substrate, in 24 well multiwell dishes

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12.6) synthesized in this way in the cytosol are transported into the vacuole by a specific glutathione translocator against a concentration gra-dient