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Precursors exceeding a defined intensity threshold were isolated (isolation window 0.4 m/z) and fragmented (nCE: 18% for soto-p 7 /p 8 , 28% for ada-p 7 , 28% for p 7 /p 8 , 20% for diva-p 7 ) with a scan range of 150-1,700 m/z, 22,500 resolution, 43 second max IT, AGC target of 5e 5