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Briefly, the storage solution was removed (5000 g , 2 min), and columns were washed with CH3CN (2 ×300 μL, 5,000 g , 2 min), then equilibrated with buffer A (2 × 300 μL, 5000 g , 2 min)

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Posttranslational modifications, like phosphorylation, or the interaction with other proteins appears to be more important for the activation of EneA