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Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY −log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated (Pham et al., 2020)
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The therapeutic effects of antidepressants consist in the increased function of the noradrenergic or serotonergic system, leading to increased activity of transcription factor CREB (cAMP response element binding protein), higher expression of neurotrophin BDNF and its receptor trkB, and consequently to increased neuronal plasticity and resumption of cellular functions