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Fixed cells were washed with PEM buffer (100 mM PIPES pH 6.9, 1 mM MgCl 2 , and 1 mM EGTA), incubated in PEMS buffer (100 mM PIPES pH 6.9, 1 mM MgCl 2 , 1 mM EGTA, and 1 M sorbitol) at room temperature for 5 min, and permeabilized with 1 mg/mL Zymolyase 100 T (Seikagaku) in PEMS buffer at 37 °C for 30 min

use:hydration

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[DOI] [PMC free article] [PubMed] [Google Scholar] 168.Dumont M., Kipiani K., Yu F., Wille E., Katz M., Calingasan N.Y., Gouras G.K., Lin M.T., Beal M.F