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Serum obtained after centrifugation were analyzed with Alanine aminotransferase Assay Kit (C009-2-1), Aspartate aminotransferase Assay Kit (C010-2-1), Direct bilirubin (D-BIL) kit (C019-2-1), Total bilirubin (T-BIL) Kit (C019-1-1), Alkaline phosphatase Assay kit (A059-2-2), Total bile acid Assay kit (E003-2-1), Glutathione Assay kit (A005-1-2), Malondialdehyde Assay kit (A003-1-2) from Nanjing Jiancheng Bioengineering Institute (Nanjing, China)