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The next day, the bacteria were centrifuged at 6,000 rpm for 5 min at 4 °C, followed by incubation of the pellets in bacterial lysis buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 5 mM imidazole, 0.2 mM TCEP, 0.5 mM PMSF, 5% glycerol and complete protease inhibitor) for 60 min on ice

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You do not want to pause mid-reconstitution to hunt for supplies