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Measurement of glutathione peroxidase and glutathione reductase activity To measure GPx activity, 120μL of saliva were incubated in a final volume of 3mL with phosphate-EDTA buffer (100mM, pH 7.5, EDTA-Na2 1mM) in the presence of 30μL of NADPH (20mM), 100μL of GSH (60mM), and 4μL (1IU) of GRd for 5min at room temperature

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doi: 10.1016/S0006-8993(98)00292-3