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While the RNI for maintenance is only 1.5 µg daily, therapeutic oral doses typically range from: 50-150 µg daily for mild deficiency prevention in at-risk groups 1,000-2,000 µg daily for treating confirmed deficiency without malabsorption Some formulations contain up to 5,000 µg, though there is no proven additional benefit over 1,000-2,000 µg for most patients The reason for this dosage discrepancy lies in absorption efficiency

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Large cell clusters contained as many as 60 cells within herniated discs (typically 5–30 cells)