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We could express a protease of interest under an inducible promoter and select in the presence of chloramphenicol in Escherichia coli expressing a large library of peptide substrates present in the linker between T7 RNAP and its fused T7 lysozyme inhibitor

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The relevant published cosmetic studies showing human skin improvement (such as the split-face studies cited in Leyden and colleagues' review of copper peptides in cosmetics) used concentrations in the low parts-per-million range, meaning micrograms per milliliter