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Chemicals and Reagents Dulbecco’s Modified Eagle’s Medium (DMEM), (with 4.5 g/L glucose/L-glutamine/sodium bicarbonate/sodium pyruvate, liquid, sterile-filtered) (Invitrogen #11995-073), Dulbecco’s Modified Eagle’s Medium (DMEM), no phenol red (Invitrogen #21063-029), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 HEPES (DMEM/F-12) (Invitrogen #11330-057), Dulbecco’s Phosphate Buffered Saline (PBS) 1× (with MgCl and CaCl 2 ) (Invitrogen #14040-182), Foetal bovine serum (FBS) (Invitrogen #26140-079), horse serum (HS) (Invitrogen #16050-122), trypsin-EDTA 10× (Invitrogen #15400-054), penicillin-streptomycin (P/S) solution (5,000 U/mL) (Invitrogen #15070-063), and page ruler prestained protein ladder (Invitrogen #26617) were purchased from ThermoFisher Scientific (Brisbane, Australia), unless otherwise stated

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