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Cell suspensions were lysed by freeze-thawing, and PV VLPs were purified from clarified supernatants by concentration through a 30% sucrose cushion followed by a 15–45% sucrose gradient in 1× Dulbecco’s phosphate-buffered saline (DPBS, Gibco), 20 mM EDTA, pH 7.0 (DPBS-EDTA) 15

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TNRF2 can induce tumoral and neuronal cell death by binding mTNF-α