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According to the instructions of the Annexin V-FITC Apoptosis Detection Kit (Beyotime, C1062), 5 μL of Annexin V-FITC reagent was added and gently mixed, followed by the addition of 10 μL of propidium iodide staining solution, which was also gently mixed

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Studies show that azelaic acid helps decrease visible redness, though more research can be conducted to show just how the ingredient achieves this result