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Protein precipitation using methanol (Merck KGaA, Darmstadt, Germany) and 0.1% formic acid (Merck KGaA, Darmstadt, Germany) was performed and the collected supernatant was treated with a derivatization solution of aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) (Merck KGaA, Darmstadt, Germany)

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[DOI] [PubMed] [Google Scholar] 73.Hartmann R.M., Fillmann H.S., Isabel M., Martins M., Meurer L., Marroni N.P