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For 96-well plates, 10 µL of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturer’s instructions) was added to wells containing 100 µL of culture media and incubated for 30 min at 37 °C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)

Surgical procedures

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Preparing your injection Remove the reconstituted KLOW vial from refrigeration