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Researchers prepare fresh aliquots for each experimental run, document concentration calculations carefully, and confirm peptide identity by mass spectrometry prior to use in formal study protocols

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Commonly used points include Ashi points (non-fixed points that are selected based on tenderness or pain), Jiaji (EX-B2), GB34 (Yanglingquan), LI4 (Hegu), ST36 (Zusanli), GB21 (Jianjing), LR3 (Taichong), SP6 (Sanyinjiao), BL15 (Xinshu), and PC6 (Neiguan) [64, 65, 66, 67, 68, 69]