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Antitumor effect of malaria parasite infection in a murine Lewis lung cancer model through induction of innate and adaptive immunity

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Generation of stable knockdown cell lines To generate RAW264.7 or HEK293T cells stably knockdown Klhl9 , Klhl13 or Cul3 , the lentiviral expression pLKO.1-TRC cloning vector encoding shRNA for Klhl9 , Klhl13 or Cul3 were co-transfected together with the lentiviral packaging plasmids psPAX2 and pMD2.G in HEK293T cells using lipofectamine 3000 (Invitrogen, L3000015) respectively according to the manufacturer’s instructions