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Because estrogen stimulates the endogenous synthesis of phosphatidylcholine via the phosphatidylethanolamine N-methyltransferase (PEMT) pathway, premenopausal women may be less likely to develop signs of choline deficiency in response to a low-choline diet compared to postmenopausal women (18, 19)

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General cell culture and imaging The HeLa cell line used in this study was purchased from American Type Culture Collection (ATCC) and grown in DMEM (Gibco, 11965) media supplemented with 10% FBS and 1% 1003 Pen Strep (Gibco)

It is produced throughout the body and is one of the bodys most important intracellular antioxidants