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Cells harvested at 0 h were centrifuged again (200 × g , 5 min, RT) immediately after resuspension and lysed using 1 ml Trizol

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With the sequence Met-Glu-His-Phe-Pro-Gly-Pro, Semax represents a modified ACTH fragment structurally designed to enhance peptide stability and resistance to enzymatic degradation compared to native ACTH sequences