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Detection of S-glutathionylated FABP5 Cells were lysed in NP-40 lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA] containing 20 mM N -ethylmaleimide (Sigma-Aldrich, E1271) and protease inhibitor cocktail (Roche, 04693132001)

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Although both CB1 and CB2 receptors are present in the retina, the neuroprotective activity of eCBs has been investigated in various models of RGC pathology and seems to be related to CB1 receptor (Kokona et al., 2016)