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However, given that previous demonstrations of longevity resulting from disruption of this pathway occurred either at conception or in young adulthood 6,7,17,18,19 , along with the reported importance of low exposure to GH (and IGF-1) signals early in life on longevity and related outcomes 20,21 , whether benefits can be achieved by targeting this pathway later in life is unclear

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Gels were stained with Coomassie or blotted onto nitrocellulose membranes, stained with Ponceau S and subjected to autoradiography