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2.5 Intracellular cAMP quantification Cyclic AMP levels were assessed using the cAMP-Glo™ Assay (V1501, Promega), according to the manufacturer's instructions

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Start retatrutide at 1mg regardless of your previous tirzepatide dose, allow a 7-10 day washout period after your last tirzepatide injection, and follow the standard retatrutide escalation schedule