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Protein-matched aliquots from lysates of HEK293 cells transfected with ABHD5 (or ABHD5 mutant)-luciferase(N) or PLIN1- or PLIN5-luciferase(C) were incubated together with increasing half-log doses of ABHD5 ligand SR4995 for four hrs at room temperature, and luminescence read after adding coelenterazine substrate